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Original Research Article | OPEN ACCESS

MiR-449b-5p modifies glycolysis by lactate dehydrogenase A in gastric cancer

Yu Gao, Jingwang Ye, Dewen Tan, Haode Shen, Li Wang

For correspondence:-  Li Wang   Email: liwang516@yeah.net   Tel:+862368757958

Accepted: 16 April 2021        Published: 30 May 2021

Citation: Gao Y, Ye J, Tan D, Shen H, Wang L. MiR-449b-5p modifies glycolysis by lactate dehydrogenase A in gastric cancer. Trop J Pharm Res 2021; 20(5):911-916 doi: 10.4314/tjpr.v20i5.4

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the role of miR-449b-5p in gastric cancer (GC) metabolism.
Methods: Human GC samples and their corresponding normal tissues were used in this study. Cell survival ability was evaluated using a commercial MTT kit. Glucose uptake, lactate production and ATP content were assessed using commercial kits. The expression levels of miRNAs were assessed by stem-loop reverse transcription-polymerase chain reaction (RT-PCR).
Results: MiR-449b-5p expression decreased in both GC tissues and cells. When the cells with low level of miR-449b-5p were transfected with miR-449b-5p mimics, glycolysis was inhibited (p < 0.05). Lactate dehydrogenase A (LDHA) was predicted as a target gene of miR-449b-5p and verified using luciferase reporter assay. MiR-449b-5p expression was up-regulated in GC cells (p < 0.05). Furthermore, miR-449b-5p expression was related to long overall survival time in patients with GC.
Conclusion: The findings demonstrate that miR-449b-5p reverses the glycolytic state of GC cells by targeting LDHA expression, and thus, it can potentially be developed for the treatment of gastric cancer

Keywords: Gastric cancer, Lactate dehydrogenase A, MiR-449b-5p

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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